Beilstein J. Nanotechnol.2016,7, 138–148, doi:10.3762/bjnano.7.16
.7.16 Abstract DNAhandles are often used as spacers and linkers in single-molecule experiments to isolate and tether RNAs, proteins, enzymes and ribozymes, amongst other biomolecules, between surface-modified beads for nanomechanical investigations. Custom DNAhandles with varying lengths and chemical
end-modifications are readily and reliably synthesized en masse, enabling force spectroscopic measurements with well-defined and long-lasting mechanical characteristics under physiological conditions over a large range of applied forces. Although these chemically tagged DNAhandles are widely used
under varying physiological conditions are lacking in literature. Here, optical tweezers are used to investigate different protein-labelled DNAhandles in a microfluidic environment under different physiological conditions. Digoxigenin (DIG)-dsDNA-biotin handles of varying sizes (1000, 3034 and 4056 bp
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Figure 1:
Bead arrangements in different optical force measurements. a) PDHs tethered to an anti-DIG bead (2....